Jan Larsson

Chromosome-wide gene regulation in Drosophila

Chromosomal instability which involves the deletion and duplication of chromosomes or chromosome parts is a common feature of cancers. Still, how regulation of whole-chromosomes or large-chromosome-domains is affected by deficiencies or chromosome loss (e.g. somatic elimination) is largely unknown. We believe that our chromosome 4 model is very suitable to study domain and whole chromosome gene regulation. Our main goal is to contribute to the understanding of targeting, function and evolution of chromosome-wide gene regulation. In particular, we focus on exploring autosome-specific gene regulation by the continued study of the protein POF (Painting of Fourth) in Drosophila.

Chromosome-wide targeting have until recently been consider only as a mechanism to equalize the transcriptional activity of the single male X-chromosome with that of the two female X-chromosomes, i.e., dosage compensation. Our discovery of the chromosome specific protein Painting-of-Fourth (POF) is the first example of a chromosome-wide targeting mechanism for an autosome. We have recently shown that POF and heterochromatin-protein-1 (HP1) are involved in the global regulation of the 4th chromosome in Drosophila. POF binding is dependent on heterochromatin and POF and HP1 bind interdependently to the 4th chromosome. We have proposed a balancing mechanism involving POF and HP1 that provides a feed-back system for fine-tuning expression status on the 4th chromosome. Balancing mechanisms may be a general way to regulate gene expression at a chromosome-wide level. The continued analysis of chromosome-wide regulation focuses on three different questions:

1. Generality, function and mechanisms for chromosome targeting and regulation.
2. Establishment and propagation of chromatin structure within and between chromosomes.
3. Evolution of chromosome-wide targeting mechanisms.


At present, POF is likely to represent the most compelling example of a chromosome targeting mechanism adapted for targeting of regulatory factors to an autosome. We believe that our work on POF has the potential to make major contribution to our understanding of chromosome-wide regulatory complexes, their composition, function and evolution.

Publications

Author

Title

Year sorteringsordning

Fulltext

Lundberg, Lina E
Kim, Maria
Johansson, Anna-Mia; et al.

Targeting of painting of fourth to roX1 and roX2 proximal sites suggests evolutionary links between dosage compensation and the regulation of the 4th chromosome in Drosophila melanogaster
G3, 3(8): 1325-1334

2013

Download

Lundberg, Lina E
Stenberg, Per
Larsson, Jan

HP1a, Su(var)3-9, SETDB1 and POF stimulate or repress gene expression depending on genomic position, gene length and expression pattern in Drosophila melanogaster
Nucleic Acids Research

2013

Download

Crona, Filip
Dahlberg, Olle
Lundberg, Lina E; et al.

Gene regulation by the lysine demethylase KDM4A in Drosophila
Developmental Biology, 737(2): 453-463

2013

-

Figueiredo, Margarida L A
Philip, Philge
Stenberg, Per; et al.

HP1a Recruitment to Promoters Is Independent of H3K9 Methylation in Drosophila melanogaster
PLoS genetics, 8(11): e1003061-

2012

Download

Johansson, Anna-Mia
Stenberg, Per
Allgardsson, Anders; et al.

POF regulates the expression of genes on the fourth chromosome in Drosophila melanogaster by binding to nascent RNA
Molecular and Cellular Biology, 32(11): 2121-2134

2012

-

Lundberg, Lina E
Figueiredo, Margarida L A
Stenberg, Per; et al.

Buffering and proteolysis are induced by segmental monosomy in Drosophila melanogaster
Nucleic Acids Research, 40(13): 5926-5937

2012

Download

Johansson, Anna-Mia
Allgardsson, Anders
Stenberg, Per; et al.

msl2 mRNA is bound by free nuclear MSL complex in Drosophila melanogaster
Nucleic Acids Research, 39(15): 6428-6439

2011

-

Stenberg, Per
Larsson, Jan

Buffering and the evolution of chromosome-wide gene regulation
Chromosoma, 120(3): 213-225

2011

-

Stenberg, Per
Lundberg, Lina E
Johansson, Anna-Mia; et al.

Buffering of segmental and chromosomal aneuploidies in Drosophila melanogaster
PLoS genetics, 5(5): e1000465-

2009

-

Johansson, Anna-Mia
Stenberg, Per
Pettersson, Fredrik; et al.

POF and HP1 bind expressed exons, suggesting a balancing mechanism for gene regulation
PLoS Genet, 3(11): e209-

2007

-


Page Editor: Jan Larsson
2010-09-08

Print page

Contact Information

Department of Molecular Biology
Umeå University
901 87 Umeå 

Visiting Address
6K och 6L, Sjukhusområdet, 6L-247

Tel:  +46 (0)90-7856785

Contact Form